Organic VS Non Organic

(Article from Soil Association).

According to Carlo Leifert, professor of ecological agriculture at Newcastle University who led the study, “The organic versus non-organic debate has rumbled on for decades now but the evidence from this study is overwhelming – that organic food is high in antioxidants and lower in toxic metals and pesticides.”

Clear differences between organic and non-organic food
Organic food has more of the antioxidant compounds linked to better health and lower levels of toxic metals and pesticides, according to the most comprehensive scientific analysis from a team at the University of Newcastle at team, led by Prof Carlo Leifert. The international scientific team behind the new work suggests that switching from regular to organic fruit and vegetables could give the same benefits as adding one or two portions of the “five a day” currently recommended. Helen Browning, Soil Association Chief Executive commented: “The crucially important thing about this research is that it shatters the myth that how we farm does not affect the quality of the food we eat. This research backs up what people think about organic food. In other countries there has long been much higher levels of support and acceptance of the benefits of organic food and farming. We hope these findings will bring the UK in line with the rest of Europe.”
The Guardian (11 July 2014)
New study finds significant nutritional differences between organic and non-organic food Countryfile Online (11 July 2014)
New study shows clear differences between organic and non-organic food The Daily Telegraph (11 July 2014)
New study shows clear differences between organic and non-organic food The Independant (11 July 2014)
Study of organic crops finds fewer pesticides and more antioxidants The New York Times (11 July 2014)
Switching to organic equivalent to getting up to two extra five-a-day portionsNatural Products Online (11 July 2014)
Will eating organic food make you healthier? The Guardian (11 July 2014)
Study sparks organic foods debate BBC News (11 July 2014)
Study finds organic produce is more nutritious Nature.com (11 July 2014)
Organic Choices are more Nutritious and Possibly Healthier Science World Report (13 July 2014)
Study of organic crops finds more antioxidants Good food (14 July 2014)
Study: Organic food has more antioxidants, less pesticide residue Huffington Post (12 July 2014)
Study: Organic produce has fewer pesticides, more antioxidants Time Magazine(12 July 2014)
Clear differences between organic and non-organic food, study finds The Times of India (12 July 2014)
Major study documents benefits of organic farming Washington State University News (11 July 2014)
Read our full press comment here

 

 

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The in vitro effect of manuka honeys on growth and adherence of oral bacteria

The in vitro effect of manuka honeys on growth and adherence of oral bacteria

smile-906956-m

Abstract

Honey has been used since ancient times and more recently, for the healing of wounds and against infectious diseases. The aim of our study was to investigate the effect of two manuka honeys showing different potencies of their antibacterial activity, on potentially pathogenic oral bacteria.

The antimicrobial activity was examined by determining the MIC and MBC using the macro dilution broth technique. The effect on the adherence was tested on growing cells of Streptococcus mutans on a glass surface and on a multi-species biofilm grown on saliva-coated hydroxyapatite discs.

As expected, the antibacterial activity of manuka Image (with higher potency of antibacterial activity) was the most important. The two tested honeys weakly inhibited the adherence of S.mutans cells to a glass surface at sub-MIC concentration.

Manuka Image showed a total inhibition of multi-species biofilm at the concentration of 200 μg/ml manuka Image inhibited biofilm formation weakly at the concentration of 200 μg/ml but firmly at the concentration of 500 μg/ml.

Our findings suggest that manuka honeys might be able to reduce oral pathogens within dental plaque. These two honeys appear to be able to control dental biofilm deposit.

Full research HERE.

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Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand

Professor Thomas Henle and colleagues at The Technical University of Dresden publish the research results upon identifying the substance formerly known as the “unique manuka factor” primarily responsible for the non-peroxide activity of manuka honey.

January 2008
Molecular Nutrition Food Research


 

1 Introduction

The use of honey as a traditional remedy for bacterial infections is known since ancient times. According to [1], scientific research in that field aiming at the identification of antibacterial compounds started with the pioneering reports of van Ketel in 1892. Dold et al. [2] established the term “inhibine” for the light and temperature sensitive antibacterial substances in honey without further chemical characterization. Since then, numerous investigations have been undertaken in order to explain the antibacterial activity of individual honey samples by osmotic effects, or the low pH value caused by several organic acids [3–6]. The most known inhibine is hydrogen peroxide [7, 8], which is formed in the honey by glucose oxidase. Several studies, however, have shown that certain honey samples possess an antibacterial activity which persists after removal of hydrogen peroxide by catalase [8]. It has been observed that Manuka honey, derived from the Manuka tree (Leptospermum scoparium) in New Zealand, has a very high level of “nonperoxide” antibacterial activity [9–10]. The pronounced antibacterial activity of Manuka honey is an important commercial property, which is referred to in marketing purposes as the so-called “Unique Manuka Factor” (UMF), leading to a classification of premium products based on microbiological assays [11].

Although several studies have been undertaken in order to characterize the components responsible for the “non-peroxide” antibacterial activity of Manuka honey [12], the chemistry behind this phenomenon still remains unclear. In a recent report, Weigel et al. [13] showed that honey contains varying amounts of 1,2-dicarbonyl compounds such as glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosulose (3-DG) besides 5-hydroxymethylfurfural, a wellknown indicator for heat-treatment. 1,2-Dicarbonyl compounds are formed in the course of the Maillard reaction or caramelization reactions as degradation products from reducing carbohydrates [14]. Motivated by a report from Suortti and Mlkki [15], who found that heated solutions of glucose or fructose, respectively, exhibit a pronounced antibacterial activity against Escherichia coli (E. coli), we investigated the hypothesis whether 1,2-dicarbonyl compounds present in Manuka honey may be related to the nonperoxide activity of these food items. 1,2-Dicarbonyl compounds were quantified after derivatization with orthophenylenediamine as the corresponding chinoxalines using RP-HPLC with UV-detection. Antibacterial properties of individual 1,2-dicarbonyls as well as of honey samples were evaluated using an agar well diffusion assay.

 

2 Materials and methods

2.1 Chemicals

GO and MGO, each 40% in water and MGO, were from Sigma (Taufkirchen, Germany). MGO was purified according to [16] with additional redistillation [17]. 3-DG was synthetized according to [18] with some modifications [19]. Acetic acid p.a. was obtained from BioChemica (Darmstadt, Germany). Phosphoric acid (85%, p.a.) was from Merck (Darmstadt, Germany). Orthophenylendiamine (OPD) was obtained from Fluka (Munich, Germany). HPLC grade methanol was from Riedel de Haen (Seelze, Germany). Water used for preparation of buffers and solutions was obtained with a Purelab plus purification system (USFilter, Ransbach-Baumbach, Germany). Agar agar, yeast extract, peptone, tryptone, and sodiumchloride were applied from AppliChem (Darmstadt, Germany).

2.2 Honey samples

A total of 50 honey samples of various origin were obtained from local retail stores or were received as a gift from Beekeeper’s association Dresden (Imkerverein Dresden, e.V.), respectively. The six samples of Manuka honey were ordered via the internet from suppliers of New Zealand honey.

2.3 Analytical RP-HPLC

1,2-Dicarbonyl compounds were analyzed as the corresponding quinoxalines after derivatization with OPD according to [13] with some slight modifications. 1.0 mL of 15% w/v solutions of honey in 0.5 M sodium phosphate, pH 6.5, or standard solutions of the 1,2-dicarbonyl compounds were mixed with 0.6 mL of a 1.0% w/v solution of OPD in 0.5 M sodium phosphate buffer, pH 6.5. The mixturewas kept in the dark for 12 h at room temperature. After membrane filtration (0.45 lm), 20 lL of this samples were subjected to chromatography analysis. This was performed using an kta Basic System with a pump P-900, UV monitor UV-900 and an auto sampler A-900 (all from Pharmacia, Freiburg, Germany). A stainless steel column, 250 mm64.6 mm, filled with Eurospher 100 RP18-material of 5 lm particle size (Knauer, Berlin, Germany), was used. The flow rate was 0.8 mL/min. The column temperature was set at 308C. The mobile phases were 0.15% acetic acid (solvent A) and 80% methanol containing 20% solvent A (solvent B). The gradient started with 20% solvent B over a period of 2 min, then it was changed linearly to 40% solvent B over a period of 20 min and to 100% solvent B within 15 min, followed by an elution with 100% solvent B over a period of 5 min and then it was changed to 20% solvent B in 7 min with subsequently equlibration with 20% solvent B for 5 min. Peaks were detected by measurement of UV-absorbance at 312 nm. External calibration using reference compounds in the range from 10 to 500 mg/L for 3-DG, from 0.1 to 20 mg/L for GO and from 0.1 to 300 mg/L for MGO was performed. All calibration curves showed linearity within these concentration ranges. Detection limits were 0.3 mg/kg for 3-DG and 0.2 mg/kg for GO or MGO, respectively.

2.4 LC/mass spectroscopy

LC-MS measurement was performed with a LC system 1100 Series (Agilent Technologies, PaloAlto, USA) and a Mariner ESI-TOF mass spectrometer (PerSeptive Biosystem, Framingham, USA). Chromatographic conditions were as above. Electrospray ionization was performed in the positive ionization mode. Nitrogen was used as curtain gas (1.5 L/min) and nebulizer gas (0.8 L/min). The mass spectrometer operating conditions were as follows: spray tip potential 5190 V, SCIEX heater 2908C, nozzle potential 90 V, skimmer 1 potential 11.50 V, quadrupole DC potential 7.50 V, deflection voltage –1.00 V, einzel lens potential –34 V, quadrupole RF voltage 1000 V, quadrupole temperature 1408C, nozzle temperature 1408C, push pulse potential 545 V, pull pulse potential 210 V, pull bias potential 3 V, acceleration potential 4000 V, reflector potential 1550 V, and detector voltage 2250 V. Full scan mass spectra were measured in mass range 100–3000 m/z in the tic-mode. The instrument was calibrated using a protein mixture containing bradykinin, angiotensin I, and neurotensin (Sigma–Aldrich, Steinheim, Germany). Data acquisition and handling was preformed using the software Data Explorer Version 4.0.0.1 (Applied Biosystems, Foster City, USA).

Table 1. 1,2-Dicarbonyl compounds and HMF in honey samples and one pharmaceutical preparation (data given in mg/kg as median, minimum and maximum value; for samples of Manuka honey data are mean l SD resulting from triplicate analysis; abbreviations are as follows: 3-DG, 3-deoxyglucosulose; GO, glyoxal; MGO, methylglyoxal; HMF, 5-hydroxymethylfurfural; n.d., not detectable, below 0.2 mg/kg; n.a., not analyzed)

Table 1
Table 1

2.5 Assessment of antibacterial activity

Antibacterial activity of honey and solutions of 1,2-dicarbonyl were analyzed using an agar well diffusion assay according to Patton et al. (2005) [20]. E. coli or Staphylococcus aureus (S. aureus) were precultivated overnight at 378C in 50 mL flasks containing 10 mL of nutrient broth according to [21]. Afterwards 0.1 mL of the cultures were spreaded on plates containing solidified nutrient medium. Wells 10 mm in diameter (0.2 mL capacity) were bored into the surface of the agar medium. 0.15 mL of solutions of the 1,2-dicarbonyl compounds GO, MGO, and 3-DG in 0.5 M sodium phosphate buffer, pH 6.5, or honey diluted to concentrations ranging from 15 to 80% in the same buffer were placed into the wells. Plates were incubated at 378C for 20 h. The zones of inhibition were measured. MIC values (minimum inhibitory concentration) were determined. MIC corresponds to the lowest concentration, for which an inhibition zone was visually detectable.

 

3 Results

The trapping of 1,2-dicarbonyl compounds with orthophenylenediamine and subsequent chromatographic analysis of the corresponding chinoxalines using RP-HPLC with UV detection at 312 nm is a generally accepted method for the quantification the degradation compounds formed from carbohydrates during Maillard reactions or caramelization. Recently, we were able to quantify 1,2-dicarbonyls in honey [13] for the first time. In the present study, a large number of commercially available samples were investigated and it could be shown, that for most of the conventional honeys, the amount of GO and MGO was low when compared to 3- DG (Table 1). GO and MGO did not exceed maximum levels of 5 mg/kg and were not affected by storage conditions, whereas up to 1451 mg/kg of 3-DG were measured. The observation by Weigel et al. [13] that no correlation exists between the amount of HMFand 3-DG, the latter representing the direct precursor for HMF, could be confirmed.

Table 2. MIC (minimally inhibitory concentration) of solutions of 1,2-dicarbonyls or diluted honey samples, respectively, and concentrations of MGO in honey samples diluted to corresponding MIC

Table 2

While analyzing further honey samples, we noticed for samples of commercially available Manuka honey from New Zealand (Fig. 1A) surprisingly high peaks of a chinoxaline eluting with identical retention time as the chinoxaline formed from MGO and orthophenylenediamine (Fig. 1B). Using a photodiodearry detector, it could be shown that the UV-spectra of the peaks detected in the honey samples were identical to that in the reference sample of MGO. For unambiguous identification of the chinoxaline derived from MGO, LC-TOF-MS was performed. Identical mass spectra with a dominant signal at an m/z of 145.1 when measured as [MH]+ were found for the peak eluting at 39 min in the chromatograms of Manuka honey samples as well as the reference sample of MGO (Fig. 2). This clearly proves the unambiguous identification of the chinoxaline formed from MGO and orthophenylendediamine. In six samples of Manuka honey from New Zealand, concentrations for MGO ranged from 38 to 761 mg/kg (Table 1), which is up to 1000-fold higher than corresponding data for the conventional honey samples. Interestingly, there was an indication that the “UMF-value”, which is a commercially used parameter to rate the antibacterial activity of Manuka honey, is directly related to the content of MGO (Table 1).

Figure 1
Figure 1
Figure 2
Figure 2

Based on that surprising result, we hypothesized that the antibacterial activity of Manuka honey may at least in part be due to MGO. In order to prove this hypothesis, first the antibacterial properties of the 1,2-dicarbonyl compounds were evaluated using an agar well diffusion assay. As can be seen from Table 2, all compounds studied exhibited an inhibiting effect against E. coli and S. aureus. The pronounced antibacterial effect was found for MGO, which is expressed by a MIC value of 1.1 mM for both bacteria strains. The values for MIC represent the minimum concentration of a compound for which an inhibiting effect was detectable. GO, for which a MIC of 6.9 mM for E. coli and 4.3 mM for S. aureus were measured, and 3-DG, which showed no inhibition at concentrations up to 60 mM, were significantly less effective inhibitors for bacterial growth when compared to MGO (Table 2). Next, it was evaluated whether honey samples exhibit an antibacterial effect under the conditions used in our assay. A pronounced antibacterial activity was only found for the samples designated as “active Manuka honey”. For this samples, MIC values, expressed as concentrations after dilution in 0.5 M phosphate buffer, ranging from 15 to 30% were measured. All other honeys did not show any antibacterial effect in dilutions below 80%. Based on the quantitative data measured for MGO via RP-HPLC, it could be calculated that the amount of MGO “active” Manuka honeys diluted to the corresponding MIC, were similar to the determined MIC values of the standard solutions of the 1,2-dicarbonyl compound. In other words, diluting of Manuka honeys to concentrations between 15 and 30% resulted in concentrations of MGO from 1.1 to 1.8 mM, which are high enough to exhibit antibacterial effects.

This assumption was finally verified by adding the amount of pure MGO, which is present in a 20% solution of an active Manuka honey, to a 20% dilution of an “inactive” forest honey. This “inactive” honey did not show an antibacterial activity at a concentration of 20% (Fig. 3, sample 4), whereas for the sample of Manuka honey, an inhibition zone was clearly visible (Fig. 3, sample 1). This Manuka honey dilution contained 1.9 mM MGO. After adding 1.9 mM MGO to the 20% solution of the forest honey, an inhibition zone comparable to that of an active Manuka honey was visible (Fig. 3, sample 3). Based on this observation, it can be concluded the MGO present in Manuka honey is directly responsible for the pronounced antibacterial activity of Manuka honey.

Figure 3
Figure 3

4 Discussion

The fact that honey originating from the Manuka tree (L. scoparium) has a significantly higher level of antibacterial activity when compared to other honeys has been reported by several authors [9–11]. This antibacterial activity could not be explained solely by the enzyme glucose oxidase, which is present in honey originating from the bee, inducing the formation of hydrogen peroxide when honey is diluted [7, 12]. This additional contribution in antibacterial activity was referred to a “non peroxide activity” or “UMF”, and several attempts were made in order to identify the compounds responsible for this effect. Preliminary phenomenological studies showed that the compounds are heat and light stable, and are not affected when the pH value is shifted to values above 11 during fractionation procedures [5, 22]. Several antibacterial phenolic acids such as caffeic and ferulic acid as well as syringic and methylsiringic acid and flavonoids like quercetin, isorhamnetin, and luteolin [23–25] were identified. The concentrations of these compounds in honey, however, were far too low to cause antibacterial effects. Nevertheless, a patent published recently describes methods for the preparation of UMF fortified honey and methods for the preparation of UMF-containing fractions of honey without insight into chemical details [26].

With our findings, we unambiguously demonstrate for the first time that MGO is directly responsible for the antibacterial activity of Manuka honey. It is noteworthy that such high amounts of MGO as present in Manuka honey (Table 1) have not yet been found for any other food item. Low amounts of enzymatically formed GO and MGO were reported for fermented foods such as milk products as well as beer and wine, with concentrations ranging from 3 to 11 mg/kg [27, 28]. Furthermore, MGO is known to form during coffee roasting in amounts of 23–47 mg/mg [29]. Quantitative data for 3-DG in food are not available. At present, only speculations can be made concerning the origin of MGO in Manuka honey. A nonenzymatic formation via retro-aldolization in the course of heat- or storageinduced Maillard or caramelization reactions [14] can be excluded, as relatively low amounts of HMF were measured. HMF is a sensitive indicator for heat-treatment. MGO is known as a by-product of increased glycolysis in bacteria [30], therefore a microbiological origin of the 1,2-dicarboynl compound should be taken into account. Furthermore, a recent report [31] for the first time demonstrated the estimation of MGO level in the range of 30–75 lM in various plant species and its increase in response to salinity, drought, and cold stress conditions. Whether this may be of importance for Manuka from a botanical point of view remains to be elucidated. Further studies must also clarify whether the promising experience in wound care issues reported for Medihoney, which is a pharmaceutical preparation of Leptospermum honeys from Australia certified for wound care, may directly be due to MGO [32] (www.medihoney.com). One commercially available pharmaceutical sample of an antibacterial wound dressing contained 312 mg/kg MGO (Table 1). This concentration of MGO should be high enough to cause antibacterial effects when applied to wounds.

Finally, from the nutritional standpoint, the physiological significance resulting from the uptake of MGO and other 1,2-dicarbonyl compounds must be a topic of further investigations. MGO and glycation compounds resulting from the reaction of MGO with amino acid side chains of lysine or arginine, respectively, have been identified in vivo and are associated with complications of diabetes and some neurodegenerative diseases, although the role of these compounds in the pathogenesis of different diseases have not yet been fully understood [33–35]. Information concerning a potential toxicity of dietary MGO are rare and ambivalent, as the intake of MGO has also exerted an anticancer effect [35–37]. The physiological implications resulting from an uptake of bioactive carbohydrate degradation products and a risk-benefit analysis resulting there from must be topic of further investigations.

In conclusion, with the present investigation the occurrence of high amounts of MGO in New Zealand Manuka (L. scoparium) honey was demonstrated. MGO was identified as a bioactive compound which is responsible for the antibacterial activity of these honey samples. Studies in order to clarify the pathways for the biochemical formation of MGO in Manuka plants and honey are underway in our laboratory.

Full Article (pdf): henle_molecular_nutrition_2008

5 References

[1] Dustmann, J. H., Antibacterial effect of honey. Apiacta 1979, 14, 7–11.
[2] Dold, D., Du, D. H., Dziao, S. T., Nachweis antibakterieller, hitze- und lichtempfindlicher Hemmstoffe (Inhibine) im Naturhonig. Z. Hyg. Infekt. 1937, 120, 155–167.
[3] Dustmann, J. H., ber den Einfluss des Lichtes auf den Peroxidwert (Inhibin) des Honigs. Z. Lebensm. Unters. Forsch. 1972, 148, 263 –268.
[4] Bogdanov, S., Characterization of antibacterial substances in honey. Lebensm.Wiss. Technol. 1984, 17, 74–76.
[5] Bogdanov, S., Nature and origin of the antibacterial substances in honey. Lebensm.Wiss. Technol. 1997, 30, 748 –753.
[6] Molan, P. C., The antibacterial activity of honey. 1. The nature of the antibacterial activity. BeeWorld 1992, 73, 5–28.
[7] White, J.W., Subers, M. H., Schepartz, A., The identification of inhibine. Am. Bee J. 1962, 102, 430 –431.
[8] Adcock, D., The effect of catalase on the on the inhibine and peroxide values of various honeys. J. Apic. Res. 1962, 1, 38–40.
[9] Allen, K. L., Molan, P. C., Reid, G. M., A survey of the antibacterial activity of some New Zealand honeys. J. Pharm. Pharmacol. 1991, 43, 817 –822.
[10] Russell, K. M., Molan, P. C., Wilkins, A. L., Holland, P. T., Identification of some antibacterial constituents of New Zealand manuka honey. J. Agric. Food Chem. 1990, 38, 10 –13.
[11] Molan, P. C., Why honey is effective as a medicine. 2. The scientific explanation of its effects. Bee World 2001, 82, 22–40.
[12] Weston, R. J., The contribution of catalase and other natural products to the antibacterial activity of honey: A review. Food Chem. 2000, 71, 235–239.
[13] Weigel, K., Opitz, T., Henle, T., Studies on occurence and formation of 1,2-dicarbonyls in honey. Eur. Food Res. Technol. 2004, 218, 147 –151.
[14] Mavric, E., Kumpf, Y., Schuster, K., Kappenstein, O. et al., A new imidazolinone resulting from the resulting from the reaction of peptide-bound arginine and oligosaccharides with 1,4- glycosidic linkages. Eur. Food Res. 2004, 218, 213 –218.
[15] Suortti, T., Mlkki, Y., Antimicrobial activities of heated glucose and fructose solutions and their elucidation by high performance liquid chromatography. Food Chem. 1984, 15, 165–173.
[16] Kellum, M.W., Oray, B., Norton, S. J., A convenient quantitative synthesis of methylglyoxal for glyoxalase I assays. Anal. Biochem. 1978, 85, 586–590.
[17] McLellan, A. C., Phillips, S. A., Thornalley, P. J., The assay of methylglyoxal in biological systems by derivatization with 1,2-diamino-4,5-dimethoxybenzene. Anal. Biochem. 1992, 206, 17–23.
[18] Madson, M. A., Feather, M., An improved preparation of 3-deoxy-erythro-hexos-2-ulose via the bis(benzylhydrazone) and some related constitutional studies. Carbohydr. Res. 1981, 94, 183–191.
[19] Henle, T., Bachmann, A., Synthesis of pyrraline reference material. Z. Lebensm. Unters. Forsch. 1996, 202, 72–75. [20] Patton, T., Barrett, J., Brennan, J., Moran, N., Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey. J. Microbiol. Meth. 2006, 64, 84–95.
[21] Zimbro, M. J., Power, D. A. (Eds.), Difco and BBL Manual: Manual of Microbiological Culture Media. Becton, Dickinson and Co. Sparks,MD 2003.
[22] Molan, P. C., Russell, K. M., Non-peroxide antibacterial activity in some New Zealand honeys. J. Apic. Res. 1988, 27, 62–67.
[23] Tan, S. T., Wilkins, A. L., Molan, P. C., Holland, P. T., Reid, M., A chemical approach to the determination of floral sources of New Zealand honeys. J. Apic. Res. 1989, 28, 212 –222.
[24] Weston, R. J., Brocklebank, L. K., Lu, Y., Identification and quantitative levels of antibacterial components of some New Zealand Manuka honeys. Food Chem. 2000, 70, 427–435.
[25] Russell, K. M., Molan, P. C., Wilkins, A. L., Holland, P. T., Identification of some antibacterial constituents of New Zealand Manuka honey. J. Agric. Food Chem. 1990, 38, 10–13.
[26] Snow, M.J., Manlye-Harris, M., Farr, J.M., Unique manuka factor fortified honey. PCT Internat. Patent Appl. 2005, Patent NumberWO2005120250-A1.
[27] Bednarski, W., Jedrychowski, L., Hammond, E. G., Nikolov, Z. L., A method for the determination of alpha-dicarbonyl compounds. J. Dairy Sci. 1989, 72, 2474 –2477.
[28] De Revel, G., Bertrand, A., A method for the detection of carbonyl compounds in wine: Glyoxal and methylglyoxal. J. Sci. Food Agric. 1993, 61, 267 –272.
[29] Hayashi, T., Shibamoto, T., Analysis of methylglyoxal in foods and beverages. J. Agric. Food Chem. 1985, 33, 1090 – 1093. [30] Ferguson, G. P., Ttemeyer, S., MacLean, M. J., Booth, I. R., Methylglyoxal production in bacteria: Suicide or survival? Arch. Microbiol. 1998, 170, 209–218.
[31] Yadav, S. K., Singla-Pareek, S. L., Ray, M., Reddy, M. K., Sopory, S. K., Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. Biochem. Biophys. Res. Commun. 2005, 337, 61–67.
[32] Simon, A., Sofka, K., Wiszniewsky, G., Blaser, G.et al., Wound care with antibacterial honey (Medihoney) in pediatric hematology-oncology. Support. Care Cancer 2006, 14, 91–97.
[33] Henle, T., Protein-bound advanced glycation endproducts (AGEs) as bioactive amino acid derivatives in foods. Amino Acids 2005, 29, 313 –322.
[34] Henle, T., Miyata, T., Advanced glycation end products in uremia. Adv. Ren. Replace. Ther. 2003, 10, 321–331.
[35] Nemet, I., Varga-Defterdarovic, L., Turk, Z., Methylglyoxal in food and living organism. Mol. Nutr. Food Res. 2006, 50, 1105–1117.
[36] Kalapos, M. P., Methylglyoxal in living organisms: Chemistry, biochemistry, toxicology and biological implications. Toxicol. Lett. 1999, 110, 145–175.
[37] Ghosh, M., Talukdar, D., Ghosh, S., Bhattacharyya, N., et al., In vivo assessment of toxicity and pharmacokinetics of methylglyoxal. Augmentation of the curative effect of methylglyoxal on cancer-bearing mice by ascorbic acid and creatine. Toxicol. Appl. Pharmacol. 2006, 212, 45–58.

Abbreviations: 3-DG, 3-deoxyglucosulose; GO, glyoxal; MGO, methylglyoxal; OPD, orthophenylendiamine; UMF, Unique Manuka Factor

Copyright 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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High-throughput microbial bioassays to screen potential New Zealand functional food ingredients intended to manage the growth of probiotic and pathogenic gut bacteria

A spectrophotometric bioassay was used to screen selected food ingredients intended for development of functional foods designed to influence the growth of gut bacteria.

September 2008
International Journal of Food Science and Technology


A spectrophotometric bioassay was used to screen selected food ingredients intended for development of functional foods designed to influence the growth of gut bacteria. Dose–response profiles displaying Δgrowth, the magnitude of deviation from growth of controls, were generated for probiotics Lactobacillus reuteri, Lactobacillus rhamnosus, Bifidobacterium lactis and pathogens Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus. Ingredients were manuka honey UMF™20+(dose-dependently increased probiotics and decreased pathogens); bee pollen (biphasic growth effects against all); Rosehips and BroccoSprouts® (increased all dose-dependently); blackcurrant oil (little effect) and propolis (inhibited all strains). Ingredients were also bioassayed in pairs to assess desirable or undesirable synergistic interactions. Observed synergies included manuka honey (predominantly desirable); rosehips or BroccoSprouts® (desirable and undesirable); blackcurrant oil (desirable) and propolis (tended towards synergies reinforcing its antimicrobial effects), collectively revealing a complex web of interactions which varied by ingredient and bacterial strain. Manuka honey was particularly effective at influencing gut bacteria. The surprising frequency of undesirable synergistic interactions illustrates the importance of pre-testing potential ingredient combinations intended for use in functional foods.

Research article:

rosendale_intljfoodscitech_2008

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Research: Improving Antibiotic Activity against Wound Pathogens with Manuka Honey In Vitro

Rowena Jenkins, Rose Cooper
Centre for Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, United Kingdom

Abstract

Following the discovery of synergistic action between oxacillin and manuka honey against methicillin-resistant Staphylococcus aureus, this study was undertaken to search for further synergistic combinations of antibiotics and honey that might have potential in treating wounds.

Fifteen antibiotics were tested with and without sublethal concentrations of manuka honey against each of MRSA and Pseudomonas aeruginosa using disc diffusion, broth dilution, E strip, chequerboard titration and growth curves.

Five novel antibiotic and manuka honey combinations were found that improved antibacterial effectiveness in vitro and these offer a new avenue of future topical treatments for wound infections caused by these two important pathogens.

Full Research HERE.

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